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The objective of this study is to investigate optimum condition for laccase production. The white rot fungi Pleurotus sp. and Schizophillum sp. were studied for their abilities to produce laccase. Pleurotus sp. was proven to be excellent in producing lignin peroxidase with high specific activity. The optimum pH, agitation speed, 2,5-xylidine concentration, and temperature conditions for laccase production by Pleurotus sp. were pH 4.5, 200 rpm, 1.0 mM, and 25℃, respectively. For nitrogen sources, ammonium tartrate was good for laccase production, while 0.4% ammonium tartrate concentration showed the highest potential for laccase production. The maximum laccase activity in shaking-flask batch culture were 1,500 U/L. the maximum laccase activity in jar-fermenter culture were 1,700 U/L.
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